| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
25/09/2016 |
|
Actualizado : |
09/10/2019 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L. |
|
Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. |
|
Título : |
Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection. |
|
Fecha de publicación : |
2016 |
|
Fuente / Imprenta : |
Journal of Virological Methods, 2016, v.237, p. 14-17. |
|
DOI : |
10.1016/j.jviromet.2016.08.011 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. |
|
Contenido : |
ABSTRACT.
Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.
© 2016 Elsevier B.V. All rights reserved |
|
Palabras claves : |
DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR. |
|
Thesagro : |
CITRUS. |
|
Asunto categoría : |
-- |
|
Marc : |
LEADER 02137naa a2200301 a 4500
001 1055729
005 2019-10-09
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jviromet.2016.08.011$2DOI
100 1 $aARRUABARRENA, A.
245 $aApplication of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.$h[electronic resource]
260 $c2016
500 $aArticle history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016.
520 $aABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species. © 2016 Elsevier B.V. All rights reserved
650 $aCITRUS
653 $aDNA
653 $aPCR
653 $aPLANT VIROID
653 $aPLANT VIRUS
653 $aPURIFICATION
653 $aRNA
653 $aRT-PCR
700 1 $aBENITEZ-GALEANO, M.J.
700 1 $aGIAMBIASI, M.
700 1 $aBERTALMIO, A.
700 1 $aCOLINA, R.
700 1 $aHERNÁNDEZ-RODRÍGUEZ, L.
773 $tJournal of Virological Methods, 2016$gv.237, p. 14-17.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 31 | |
| 3. |  | GIAMBIASI, M.; RUBIO, L.; BUENAHORA, J. DNA barcoding: identificación de pulgones asociados al cultivo de pimiento. 348 (resúmen). Conferencia 12. Mesa Usina Códigos de barra de la vida. Organiza SUG (Sociedad Uruguaya de Genética). Simposio: Usina de Códigos de barra de la vida, Uruguay. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.60. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
|    |
| 15. | | GIAMBIASI, M.; RODRIGUEZ, A.; ARRUABARRENA, A.; BUENAHORA, J. First report of Coenosia attenuata (Stein, 1903) (Diptera, Muscidae) in Uruguay, confirmed by DNA barcode sequences. Notes on Geographic Distribution. Check List, 2020, 16(3): 749-752. Doi: https://doi.org/10.15560/16.3.749 Article history: Received: 14 Jan 2020 / Approved: 04 May 2020 / Published: 19 Jun 2020.
Academic editor: Kirstern Lica Follmann Haseyama.
Corresponding author: Mario Giambiasi - INIA Salto Grande, email: giambiasi7@gmail.com| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
|  |
| 19. | | ARRUABARRENA, A.; SALVO, M.; GIAMBIASI, M.; VICENTE, E.; GIMÉNEZ, G.; SPERANZA, P. Caracterización genética preliminar del Programa de Mejoramiento de Frutilla de INIA, Uruguay. MV 34 - COMUNICACIONES LIBRES - MV. MEJORAMIENTO VEGETAL In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 297| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas. |
| |
| Registros recuperados : 31 | |
|
| Expresión de búsqueda válido. Check! |
|
|
